With ReadyTector®, blocking and immunodetection are carried out simultaneously. ReadyTector® blocks and incubates the blotted membrane with the primary and secondary antibody complexes in one step. This is only possible due to the fact that ReadyTector® can block the membrane much faster and more effectively compared to standard blockers. Therefore the primary and secondary antibodies don’t bind to the membrane non-specifically. There is no non-specific binding and background. With the classic Western blotting protocol this has to be prevented by several sequential washing and incubation steps. With ReadyTector® you can save several working and washing steps compared to the classic method.

The new ReadyTector Pure consists only of the ReadyTector solution. The user must add both his primary and secondary antibodies.
This offers the possibility to work also with fluorescence-labeled antibodies or with antibodies of other species.
Otherwise everything works the same way as with the other ReadyTector kits.
The ReadyTector Pure Kit consists, like the others, of the ReadyTector solution and the 10x Wash Buffer.